ZNF750 Knockout cell line (KYSE-30)

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ZNF750 Knockout cell line (KYSE-30) Order

Catalog#

YKO-H028

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ZNF750 Knockout cell line (KYSE-30)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

ZNF750

Gene ID

79755

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

40%RPMI-1640+40%F12K+10%FBS+1% Glutamine

Accession

CVCL_E0X2(Cellosaurus)

Target region

Knockout region: Exon 2 (based on transcript ZNF750-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:KYSE-30			STR Info (Cell bank) Cell Line:KYSE-30
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	9	9		9	9
D7S820	11	11.3		11	11.3
D16S539	10	12		10	12
VWA	16	18	19.0	16	18	19.0
TH01	9	9		9	9
AMEL	X	X		X	X
TPOX	9	9		9	9
CSF1PO	10	10		10	10
D12S391	17	19
FGA	24	24
D2S1338	23	23
D21S11	28	28
D18S51	14	14
D8S1179	12	15
D3S1358	15	16
D6S1043	11	20
PENTAE	13	13
D19S433	14.2	15.2
PENTAD	12	12
D1S1656	15	16

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ZNF750 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the KYSE-30 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ZNF750 gene knockout ZNF750 Knockout cell line (KYSE-30) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

ZNF750 Knockout cell line (KYSE-30)

Catalog#

YKO-H028

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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ZNF750 Knockout cell line (KYSE-30)

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